CARBOHYDRATE ESTIMATION BY ANTHRONE METHOD PDF

Methodology for Carbohydrates Determination of total carbohydrates by anthrone method Carbohydrates are the important components of storage and structural materials in the plants. They exist as free sugars and polysaccharides. The basic units of carbohydrates are the monosaccharides which cannot be split by hydrolysis into more simpler sugars. The carbohydrate content can be measured by hydrolyzing the polysaccharides into simples sugars by acid hydrolysis and estimating the resultant monosaccharides. Principle Carbohydrates are first hydrolysed into simple sugars using dilute hydrochloric acid. In hot acidic medium glucose is dehydrated to hydroxymethyl furfural.

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Methodology for Carbohydrates Determination of total carbohydrates by anthrone method Carbohydrates are the important components of storage and structural materials in the plants. They exist as free sugars and polysaccharides. The basic units of carbohydrates are the monosaccharides which cannot be split by hydrolysis into more simpler sugars. The carbohydrate content can be measured by hydrolyzing the polysaccharides into simples sugars by acid hydrolysis and estimating the resultant monosaccharides.

Principle Carbohydrates are first hydrolysed into simple sugars using dilute hydrochloric acid. In hot acidic medium glucose is dehydrated to hydroxymethyl furfural. This compound forms with anthrone a gree colored product with an absorption maximum at nm. Prepare fresh before use. Standard Glucose: Stock — Dissolve mg in mL water. Working standard — 10mL of stock diluted to mL with distilled water. Store refrigerated after adding a few drops of toluene.

Procedure Weigh mg of the sample into a boiling tube. Hydrolyse by keeping it in boiling water bath for 3 hours with 5mL of 2. Neutralise it with solid sodium carbonate until the effervescence ceases. Make up the volume to mL and centrifuge. Collect the supernatant and take 0. Prepare the standards by taking 0, 0. Make up the volume to 1mL in all the tubes including the sample tubes by adding distilled water. Then add 4mL of anthrone reagent. Heat for eight minutes in a boiling water bath.

Cool rapidly and read the green to dark green color at nm. Draw a standard graph by plotting concentration of the standard on the X-axis versus absorbance on the Y-axis.

From the graph calculate the amount of carbohydrate present in the sample tube. More in this section.

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Methodology for Carbohydrates

Shaktim Lignin Lignin is a non-carbohydrate polymer that consists of about 40 aromatic subunits which are covalently linked. Chromatographic and Electrophoretic methods Chromatographic methods are the most powerful analytical techniques for the analysis of the type and concentration of monosaccharides and oligosaccharides in foods. They are aliphatic aldehydes or ketones which contain one carbonyl group and one or more hydroxyl groups. The crude fiber method gives an estimate of indigestible fiber in foods. The two methods most commonly used to determine carbohydrate concentration are: The solution is then filtered and the fiber is collected, dried and weighed. Crude fiber measures cellulose and lignin in the sample, but does not determine hemicelluloses, pectins and hydrocolloids, because they are digested by the alkali and acid and are therefore not collected. But they yields of color where is to form carbohydrate to carbohydrate.

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